ReverHotTaq DNA Polymerase is an artificial engineered thermostable DNA polymerase, originated from BIORONs SD Polymerase. The HotStart function with AntiTaq antibody prevents non-specific amplification and allows reaction set-up at room temperature. ReverHotTaq shows a high reverse transcriptase activity for sensitive and robust cDNA synthesis and PCR amplification. Therefore, it can be used in a one-step RT-PCR assay with only one enzyme. The enzyme also possesses strand displacement activity and high thermostability up to 93 °C , so it is suitable for RNA templates with a complex secondary structure. Both total RNA and mRNA can be used as template. ReverHotTaq allows to detect target transcripts in a single reaction with only 10 pg of total RNA using gene-specific primers.
It also provides efficient, robust and specific PCR and qPCR amplification of DNA templates. Due to the 5’ – 3’ exonuclease activity, ReverHotTaq can be used in qPCR or RT-qPCR with hydrolysis probes (e.g. TaqMan).
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ReverHotTaq DNA/RNA Polymerase 50x
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ReverTaq DNA/RNA Polymerase is a new thermostable enzyme with strand displacement and reverse transcription activity for RT-PCR and qPCR.
REF: N/A
Categories: Polymerases, qPCR, Reverse Transcription, Strand Displacement
- Description
- Additional information
Description
Description
- Strand Displacement Reactions
- Standard and Multiplex PCR/ qPCR
- Reverse Transcription
ReverHotTaq Polymerase is not recommended for Isothermal Amplification.
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Additional information
Additional information
revertag | 100 rcs |
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