SD Polymerase 50 U/µl is a novel artificial thermostable polymerase with strong Strand Displacement, but lacking 5′-3′ exonuclease activity. It possesses heat stability up to 93 °C. With SD Polymerase possibilities of Isothermal Amplification and regular PCR can be combined. The efficiency of Isothermal Amplification can be raised by initial denaturation steps to increase the accessibility of single strands. SD Polymerase is efficient for high GC-content and difficult targets. Long Range PCR can be performed with target size up to 30 kb. SD Polymerase can be used in various strand displacement reactions like LAMP or WGA, Isothermal Amplification, PCDR, library generation and standard PCR reactions. BIORONs Whole Genome Amplification Kits contain SD Polymerase.
This high concentrated variant with 50 U/µl is suitable for large scale applications or LAMP. For other applications please use SD Polymerase 10 U/µl with or without antibody.