Reverase M-MuLV

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ReveraseTM is a M–MuLV reverse transcriptase without RNase H activity.

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Description

Description

ReveraseTM is a modified enzyme purified from a plasmid in E.coli strain that directs the synthesis of Moloney Murine Leukemia virus (M-MuLV) reverse transcriptase. The enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single stranded DNA as a template. ReveraseTM has been genetically altered to remove associated RNase H activity. Removal of the RNase H activity resulted in an increase of full-length cDNA products. Subsequent amplification of cDNA is possible with Taq DNA Polymerase.

Supplied with two different buffers and additional DTT and MgCl2.

  • Reverse Transcription
  • Detection of viruses in combination with PCR amplification of cDNA
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Additional information

Additional information

reverase-size

10000 U, 50000 U

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