T7 RNA Polymerase, low dsRNA 50 U/µl is used in In-vitro transcription to incorporate NTPs into the growing mRNA chain, especially in mRNA synthesis. It is an DNA-dependent RNA polymerase and synthesizes RNA in 5’→3′ direction from linear DNA containing the T7 promoter. This enzyme is a mutant form of T7 RNA polymerase derived from E. coli expression intended for use in IVT to generate mRNA. Compared to the wild-type T7 RNA polymerase, this product generates much lower level of double-strand RNA (dsRNA) during the IVT process, which makes the mRNA less immunogenic. Either linearized plasmids or PCR products can be used as IVT templates.
With enhanced lVT performance, this product can help to make better mRNA for therapeutics.
This product comes with a 10x Transmission Buffer.