Proteinase K is a non-specific serine protease with a very high specific activity. The enzyme shows high stability and activity also in the presence of SDS, EDTA and chaotropic salts. Proteinase K is free of RNase and DNase activities and has a specific activity of 30 U/mg. The recommended working concentration for Proteinase K is 50 – 100 µg/mL in the majority of applications in nucleic acid extraction.
The lyophilized powder is stable at room temperature (15 – 25 °C) for up to 4 days. For long time storage – 20°C in storage buffer is recommended. The powder is highly soluble ( > 50 mg/ml), standard stock concentration is 20 mg/ml.
BIORON also offers Proteinase K Solution with 20 mg/ml.