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Proteinase K is a broad-spectrum protease for nucleic acid purification. Free of RNase/ DNase.

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Proteinase K is a non-specific serine protease with a very high specific activity. The enzyme shows high stability and activity also in the presence of SDS, EDTA and chaotropic salts. Proteinase K is free of RNase and DNase activities and has a specific activity of 30 U/mg. The recommended working concentration for Proteinase K is 50 – 100 µg/mL in the majority of applications in nucleic acid extraction.

The lyophilized powder is stable at room temperature (15 – 25 °C)  for up to 4 days.  For long time storage – 20°C in storage buffer is recommended. The powder is highly soluble ( > 50 mg/ml),  standard stock concentration is 20 mg/ml.

BIORON also offers Proteinase K Solution with 20 mg/ml.

Isolation of nucleic acids from bacteria
Isolation/ purification of nucleic acids from cells or tissue for PCR


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Additional information

Additional information


200 mg, 1 g