Description
Ultimate TaqMan Master Mix is a ready-to-use 2x qPCR master mix optimized for highly sensitive probe-based real-time PCR, TaqMan assays, and multiplex qPCR applications. The advanced formulation combines HotStart DNA polymerase, dNTPs, MgCl₂, and an optimized reaction buffer to deliver exceptional specificity, sensitivity, and reproducibility across a broad range of quantitative PCR workflows.
The master mix is specifically optimized for hydrolysis probe-based assays such as TaqMan® qPCR and supports reliable detection of low-copy targets, complex genomic DNA, and challenging templates, including GC-rich regions. The optimized buffer chemistry minimizes non-specific amplification and primer-dimer formation while ensuring robust fluorescence signal generation and accurate quantification.
Only primers, probes, and template DNA need to be added, significantly reducing pipetting steps and minimizing the risk of contamination. This streamlined workflow improves reproducibility and makes the master mix ideal for routine qPCR, molecular diagnostics research, pathogen detection, gene expression analysis, genotyping, and multiplex assay development.
Ultimate TaqMan Master Mix supports PCR amplification of targets up to 5 kb and provides excellent performance in both singleplex and multiplex real-time PCR assays. The included HotStart polymerase remains inactive during reaction setup, enabling convenient room-temperature handling and improved assay specificity.
The formulation is designed for ice-free handling and fast cycling protocols while maintaining outstanding sensitivity and reproducibility. The master mix remains stable at room temperature for up to 14 days, simplifying storage, shipping, and everyday laboratory workflows.
