Description
Ultimate One-Step RT-PCR Master Mix is a ready-to-use 2x master mix for highly sensitive probe-based one-step RT-qPCR and RNA detection workflows. The optimized formulation combines reverse transcription and real-time PCR amplification in a single tube, enabling fast, reliable, and contamination-minimized analysis of RNA samples.
The master mix contains all components required for one-step RT-qPCR, including reverse transcriptase, HotStart DNA polymerase, dNTPs, MgCl₂, and an optimized reaction buffer. Only primers, probe, and template RNA need to be added, reducing pipetting steps and minimizing the risk of cross-contamination.
Ultimate One-Step RT-PCR Master Mix is optimized for PCR amplification of challenging RNA templates, low-copy targets, and multiplex RT-qPCR assays. The highly sensitive enzyme system delivers robust performance across a wide range of probe-based real-time PCR applications, including pathogen detection, viral RNA analysis, gene expression studies, and molecular diagnostic assay development.
The master mix is compatible with various qPCR probe chemistries and has been validated for reliable performance with RNA templates. All lots are tested in probe-based RT-qPCR assays and are free of detectable endonuclease and exonuclease activities.
For maximum convenience in laboratory workflows, the master mix is supplied as a ready-to-use liquid formulation. Selected qPCR cyclers require ROX reference dye; a ROX-containing version is available upon request.
Like all BIORON Ultimate Master Mixes, the formulation provides excellent sensitivity, high reproducibility, and outstanding stability. The mix remains stable at room temperature for up to two weeks, simplifying shipping, storage, and daily laboratory handling.
