Script III Reverase, glycerol free, 200 U/µl

Script III Reverase, glycerol free, 200 U/µl

REF: 105500GF -

Glycerol-free recombinant M-MuLV Reverse Transcriptase with reduced RNase H activity and increased thermostability for efficient first-strand cDNA synthesis. Ideal for lyophilized assays, diagnostic kit production, and One-Step RT-qPCR applications.

 

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Description

Script III Reverase, glycerol-free, is a recombinant M-MuLV Reverse Transcriptase designed for fast and efficient first-strand cDNA synthesis from RNA or single-stranded DNA templates. Genetic modifications reduce RNase H activity and increase thermostability, resulting in improved cDNA yield, specificity, and performance.

The enzyme shows optimal activity at 50–55 °C and supports the synthesis of cDNA fragments up to 13 kb. Supplied with an optimized reaction buffer and MgCl₂, it provides reliable results in reverse transcription workflows and downstream PCR applications.

The glycerol-free formulation is specifically developed for applications requiring lyophilization and is therefore particularly suitable for diagnostic kit manufacturing, assay development, and customized molecular biology workflows.

Applications

  • specifically developed for applications requiring lyophilization
  • cDNA synthesis
  • gene expression analysis
  • sequencing applications
  • One-Step RT-qPCR
  • research and diagnostic assay development requiring high-performance reverse transcription

Key Features

  • High Concentrated – for production
  • specifically developed for applications requiring lyophilization

Components

  • Script III Reverase, glycerol free
  • 5x Complete Reaction Buffer
  • MgCl2

Data Sheets