Reverase (M-MuLV, RNase H minus)

Reverase (M-MuLV, RNase H minus)

REF: 105600 - 105650

Reverase is an engineered M-MuLV reverse transcriptase for fast and efficient first-strand cDNA synthesis, RT-PCR, and RT-qPCR applications. The enzyme combines enhanced thermal stability with high sensitivity for low-copy targets and structured RNA templates.

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Description

Reverase is a genetically engineered M-MuLV reverse transcriptase developed for fast, efficient, and highly specific first-strand cDNA synthesis. The enzyme features enhanced thermal stability, allowing reverse transcription reactions at temperatures up to 60 °C, which improves the processing of RNA templates with complex secondary structures and high GC content.

Through its increased affinity for RNA templates, Reverase delivers excellent performance with low-abundance transcripts, limited sample material, and low-copy targets. The enzyme enables the synthesis of cDNA fragments up to 10 kb and provides high sensitivity and reproducibility across a broad range of reverse transcription applications.

The high purity of Reverase makes it particularly suitable for demanding workflows, including gene expression analysis, RT-PCR, RT-qPCR, next-generation sequencing sample preparation, and pathogen detection assays.

Reverase is supplied with an optimized reaction buffer to ensure robust performance, high cDNA yields, and reliable results across diverse RNA templates.

 

Applications

  • First-strand synthesis of cDNA from RNA or single-stranded DNA

 

Find our Ultimate One-Step RT-PCR Master Mix here.

 

Key Features

  • Genetically engineered M-MuLV reverse transcriptase
  • Enhanced thermal stability up to 60 °C
  • Efficient reverse transcription of structured RNA templates
  • High affinity for low-abundance RNA targets
  • Suitable for low-copy gene detection
  • cDNA synthesis up to 10 kb
  • High sensitivity and reproducibility
  • Excellent choice for RT-PCR, RT-qPCR, and pathogen detection
  • High enzyme purity for demanding applications
  • Supplied with optimized reaction buffer

Components

  • Reverase
  • 5x Complete Reaction Buffer

Data Sheets