Description
Ultimate GreenHot Master Mix is a ready-to-use 2x qPCR master mix for highly sensitive SYBR® Green-based real-time PCR applications. The optimized formulation combines antibody-blocked SuperHotTaq DNA Polymerase, SYBR® Green dye, dNTPs, MgCl₂, and an advanced reaction buffer to deliver outstanding specificity, sensitivity, and reproducibility in quantitative PCR workflows.
The integrated HotStart technology prevents polymerase activity during reaction setup, reducing non-specific amplification and primer-dimer formation. Upon initial heating, the antibody is irreversibly inactivated, enabling highly specific PCR amplification and accurate fluorescence-based quantification. This allows convenient room-temperature reaction setup while improving assay performance and data quality.
Ultimate GreenHot Master Mix is optimized for real-time PCR amplification of genomic DNA, cDNA, low-copy targets, and challenging templates, including GC-rich regions. The included SYBR® Green dye enables direct fluorescence detection during amplification without the need for sequence-specific probes, making the mix a cost-effective solution for gene expression analysis, target quantification, assay validation, and routine qPCR applications.
Only primers and template DNA need to be added, significantly reducing pipetting steps and minimizing contamination risk. The optimized buffer system supports robust amplification of DNA fragments up to 5 kb while maintaining excellent reaction efficiency and reproducibility.
Like all BIORON Ultimate Master Mixes, Ultimate GreenHot Master Mix is designed for ice-free handling and fast cycling protocols. The formulation remains stable at room temperature for up to 14 days, simplifying laboratory workflows and logistics without compromising performance.
