Description
Ultimate HotRox Master Mix is a ready-to-use 2x qPCR master mix optimized for highly sensitive probe-based real-time PCR, TaqMan® assays, and multiplex qPCR applications requiring ROX reference dye normalization. The advanced formulation combines HotStart DNA polymerase, dNTPs, MgCl₂, low-concentration ROX reference dye, and an optimized reaction buffer to ensure accurate fluorescence normalization, excellent sensitivity, and highly reproducible results.
The master mix is specifically designed for qPCR instruments that require a low ROX concentration as a passive reference dye. ROX normalization helps compensate for pipetting variations, instrument fluctuations, and well-to-well signal differences, improving data consistency and quantification accuracy across qPCR experiments.
Ultimate HotRox Master Mix is optimized for PCR amplification of low-copy targets, complex genomic DNA, GC-rich regions, and challenging templates. The HotStart polymerase remains inactive during reaction setup, minimizing non-specific amplification and primer-dimer formation while enabling convenient room-temperature handling.
Only primers, probes, and template DNA need to be added, reducing pipetting steps and minimizing the risk of contamination. The optimized buffer chemistry supports robust amplification and fluorescence detection in both singleplex and multiplex probe-based qPCR assays, making the mix ideal for pathogen detection, gene expression analysis, genotyping, molecular diagnostics research, and assay development.
The formulation supports PCR amplification of targets up to 5 kb and is particularly suitable for hydrolysis probe technologies such as TaqMan®. Like all BIORON Ultimate Master Mixes, it is designed for ice-free handling and fast cycling protocols while maintaining exceptional sensitivity and reproducibility.
