Description
SuperHotTaq DNA Polymerase, 5 U/µl is an optimized HotStart PCR enzyme consisting of recombinant Taq DNA polymerase complexed with anti-Taq monoclonal antibodies. The antibody-mediated inhibition prevents polymerase activity during PCR set-up at room temperature, reducing non-specific amplification and primer-dimer formation. Full enzyme activity is automatically restored during the initial denaturation step above 70 °C.
The enzyme efficiently amplifies DNA fragments up to 5 kb and is ideally suited for demanding PCR applications including complex genomic DNA, cDNA templates, low copy targets, multiplex PCR, and real-time PCR.
SuperHotTaq DNA Polymerase possesses a robust 5’→3’ polymerase activity and generates PCR products with 3’-A overhangs, making it compatible with standard TA cloning workflows. Optimized enzyme formulation ensures high specificity, excellent reproducibility, and clean amplification results.
For maximum flexibility in assay optimization, the polymerase is supplied with three different 10x reaction buffers:
- Incomplete NH4 buffer (without MgCl2)
- Complete NH4 buffer
- Complete KCl buffer
Additional MgCl2 solution is included for individual optimization of magnesium concentration according to specific assay requirements.
SuperHotTaq DNA Polymerase is also available in high-concentration formats suitable for lyophilization and diagnostic kit production and is used in our SuperHot Master Mix formulations.
