Description

SD Polymerase HC, 50 U/µl, is a highly concentrated artificial thermostable DNA polymerase engineered for strong strand displacement activity and exceptional performance in PCR and isothermal amplification workflows. The enzyme is stable up to 93 °C and combines strand displacement amplification with high-temperature PCR cycling in a single enzyme system.

Unlike conventional strand displacement polymerases, SD Polymerase HC retains full activity at elevated temperatures, allowing initial denaturation steps that improve template accessibility and amplification specificity. The enzyme possesses both 5’→3’ polymerase activity and 5’→3’ strand displacement activity while lacking exonuclease activity. It generates PCR products with 3’-A overhangs suitable for TA cloning applications.

SD Polymerase HC efficiently amplifies DNA fragments from 100 bp up to 30 kb and demonstrates excellent performance with GC-rich regions, low-copy targets, and complex genomic templates. The enzyme is particularly effective for PCDR, LAMP, whole genome amplification (WGA), library preparation workflows, and molecular diagnostic applications.

With a concentration of 50 U/µl, SD Polymerase HC is specifically designed for large-scale manufacturing, automated liquid handling systems, high-throughput workflows, and diagnostic kit production where highly concentrated enzyme formulations are required.