Description

SmartTaq DNA Polymerase, 5 U/µl is a genetically modified recombinant Taq DNA polymerase engineered for enhanced robustness and specificity in demanding PCR applications. The enzyme efficiently amplifies DNA fragments up to 5 kb and delivers reliable performance even in challenging assays containing inhibitor residues or requiring high multiplexing capacity.

The optimized enzyme formulation minimizes non-specific amplification and primer-dimer formation, resulting in clean and reproducible PCR products. SmartTaq DNA Polymerase is particularly recommended for multiplex PCR assays, complex sample matrices, and applications where standard Taq polymerases may be affected by PCR inhibitors.

The enzyme possesses a robust 5’→3’ polymerase activity and generates PCR products with 3’-A overhangs, making it compatible with standard TA cloning workflows.

For maximum flexibility in assay optimization, the polymerase is supplied with three different 10x reaction buffers:

• Incomplete NH₄ buffer (without MgCl₂)
• Complete NH₄ buffer
• Complete KCl buffer

Additional MgCl₂ solution is included for individual optimization of magnesium concentration according to specific assay requirements.

For applications requiring HotStart technology, SmartTaq DNA Polymerase is also available as the antibody-blocked variant SmartHotTaq DNA Polymerase.