Description

Ultimate cDNA Synthesis Kit is a complete solution for highly efficient first-strand cDNA synthesis from total RNA and mRNA templates. The kit contains all reagents required for fast, reliable, and reproducible reverse transcription, enabling high-quality cDNA generation for downstream molecular biology and gene expression applications.

The kit includes a highly efficient reverse transcriptase with reduced RNase H activity, optimized reaction buffers, dNTPs, RNase inhibitor, RNase-free water, random hexamer primers, oligo(dT) primers, and an additional DNase treatment system for efficient removal of contaminating genomic DNA prior to reverse transcription.

The optimized enzyme system enables rapid cDNA synthesis within 5–30 minutes while maintaining high sensitivity and specificity. RNA inputs of up to 10 µg per reaction can be processed, and resulting cDNA can support amplification of targets up to 17 kb. This makes the kit suitable for demanding applications involving long transcripts, low-abundance targets, and complex RNA samples.

Ultimate cDNA Synthesis Kit is optimized for downstream applications including RT-qPCR, gene expression analysis, next-generation sequencing (NGS), RNA sequencing workflows, pathogen detection, molecular diagnostics research, and cDNA sequencing.

All kit components are free of detectable endonuclease and exonuclease activity, ensuring maximum RNA integrity and highly reproducible results. The inclusion of both random hexamer and oligo(dT) primers provides maximum flexibility for reverse transcription of diverse RNA targets.

For laboratories requiring a streamlined one-tube workflow, BIORON also offers the Ultimate One-Step RT-PCR Master Mix for combined reverse transcription and real-time PCR amplification.