Description

PureColumn DNA Extraction Kit is designed for fast, reliable and high-yield purification of genomic DNA from blood, cultured cells, bacteria and complex food samples. The kit enables efficient DNA extraction from fresh or frozen blood, plasma, body fluids, mammalian cells, processed food products, milk and dairy products, as well as bacterial samples used in food testing and pathogen detection workflows.

The purification procedure is based on silica spin column technology and an optimized buffer system for efficient cell lysis, DNA binding, washing and elution. Genomic DNA is selectively adsorbed to the silica membrane while proteins, salts, inhibitors and other contaminants are efficiently removed during the washing process. The purified DNA is directly eluted in a low-salt buffer and is immediately suitable for downstream molecular biology applications.

PureColumn DNA Extraction Kit delivers high-quality genomic DNA fragments of ≥20 kb with typical A260/A280 ratios between 1.7 and 2.0. Depending on the sample type, DNA yields of 4–30 µg can be obtained from a 200 µl sample input. The kit supports extraction from blood samples with or without anticoagulants and is equally suitable for suspension and adherent cell cultures.

For food testing applications, the kit enables reliable DNA isolation from processed and preserved food samples, including meat products, sausages, salami, cheese, milk, yoghurt and milk powder. The purified DNA is suitable for PCR, qPCR, sequencing, cloning, genotyping, restriction analysis, hybridization assays, pathogen detection and NGS library preparation.