306105

Loading Buffer IV (10x)

Loading Buffer IV (10x)

REF: 306105-

Loading Buffer IV (10x) is a DNA loading dye for agarose gel electrophoresis and sample preparation. The buffer increases sample density and provides tracking dyes for reliable monitoring of DNA migration during electrophoresis.

You need to login here before you can buy any products

excl. VAT

Description

Loading Buffer IV (10x) is a concentrated DNA loading dye designed for the preparation of DNA samples prior to agarose gel electrophoresis. The buffer increases sample density, ensuring efficient loading into gel wells while providing tracking dyes for convenient monitoring of DNA migration during electrophoresis.

The formulation is optimized for DNA samples dissolved in water or TE buffer and contains bromophenol blue and xylene cyanol FF as migration markers. In a 1% agarose gel, bromophenol blue migrates approximately at the position of a 300 bp double-stranded DNA fragment, while xylene cyanol FF migrates similarly to a 4 kb DNA fragment, allowing reliable tracking of electrophoresis progress.

Loading Buffer IV also contains EDTA to protect DNA from nuclease degradation and Ficoll 400 to increase sample density for consistent loading performance. The product is supplied as a 10x concentrate and is used at a final concentration of 1x.

Composition (10x)

0.25% Bromophenol Blue
0.25% Xylene Cyanol FF
100 mM EDTA Na₂ (pH 8.0)
20% Ficoll 400
1% SDS

 

Applications

  • gel electrophoresis

Key Features

  • DNA loading dye for agarose gel electrophoresis
  • Increases sample density for reliable gel loading
  • Contains bromophenol blue and xylene cyanol FF tracking dyes
  • Suitable for DNA samples dissolved in water or TE buffer
  • Supplied as a 10x concentrate
  • Simple 1:10 dilution with DNA samples before loading
  • Stable at room temperature for up to 6 months

Components

  • Loading Buffer IV (10x)

Data Sheets


    Related products