1 kb DNA Ladder ready to use

1 kb DNA Ladder ready to use

REF: 305120

1 kb DNA Ladder, ready-to-use is a DNA size marker for agarose gel electrophoresis and DNA fragment analysis. Enhanced 1.5 kb and 4 kb reference bands enable reliable sizing of double-stranded DNA fragments from 250 bp to 12 kb.

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Description

The 1 kb DNA Ladder, ready-to-use is a convenient DNA size marker for accurate sizing of double-stranded DNA fragments from 250 bp to 12 kb during agarose gel electrophoresis. The ladder consists of 13 blunt-ended DNA fragments and covers a broad size range suitable for routine PCR analysis, cloning workflows, restriction digest verification, genomic DNA studies, and long-range PCR applications.

The 1.5 kb and 4 kb fragments are provided at increased intensity, enabling rapid orientation and easy identification of DNA fragment sizes on agarose gels. The ladder is supplied in storage buffer with loading dye included, allowing direct application to agarose gels without additional preparation steps.

An additional tube of 5x DNA Loading Buffer is included for convenient preparation of DNA samples in a final 1x concentration. The ready-to-use format reduces handling time and simplifies routine electrophoresis workflows.

The 1 kb DNA Ladder is optimized for reliable DNA fragment size determination across a wide range of molecular biology applications, including PCR product analysis, cloning, restriction digest verification, and genomic DNA analysis.

Fragment sizes (bp):
250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, 12000

 

Applications

  • gel electrophoresis

Key Features

  • DNA size marker for fragments from 250 bp to 12 kb
  • 13 blunt-ended DNA fragments
  • Enhanced 1.5 kb and 4 kb reference bands for easy orientation
  • Supplied ready-to-use with loading dye
  • Direct loading onto agarose gels without additional preparation
  • Includes 5x DNA Loading Buffer for sample preparation
  • Suitable for PCR products, restriction digests, cloning and genomic DNA analysis
  • Broad size range for routine PCR and long-range PCR applications
  • Recommended loading volume: 5 µl per lane

Components

  • 1 kb DNA Ladder ready to use
  • 5x DNA Loading Buffer

Data Sheets