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RealLine CMV

RealLine CMV

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The RealLine CMV kit is intended for the detection of CMV DNA in human samples by Real-Time PCR. The kit is designed for in vitro diagnostics and provides qualitative detection.

All components are lyophilized and ready-to use.

GLU – for General Lab Use

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Description

Description

Description

Cytomegalovirus (CMV) is a ubiquitous double-stranded DNA virus belonging to the Herpesviridae family. It is transmitted by close contact between individuals, through contamination from urine, saliva, semen, cervical secretions and breast milk. CMV can establish life-long latency in the host with possible reactivations and reinfections with heterogeneous human strains of CMV.

Sample Material

  • Blood serum, plasma
  • Saliva
  • Oropharyngeal swabs
  • Whole blood
  • Urine
  • Urogenital swabs

 

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for CMV DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881-R) seperately.

Format

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Validated Cyclers

RealLine Cycler 48 or RealLine Cycler 96 (BIORON GmbH)

CFX96, iQ5 iCycler (Bio-Rad, USA)

DT96, DTLite (DNA-Technology, Russia)

Our kits are compatibel to other Real-Time cyclers. Please read the IFU properly and ask us for recommendations of device settings!

Additional information

Additional information

RealLine HSV-1/ HSV-2

Fla, Str
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