RealLine HBV / HCV / HIV

RealLine HBV / HCV / HIV

REF: VBD0592-R

The RealLine HBV / HCV / HIV Kit the qualitative and differential detection of Hepatitis B Virus DNA, Hepatitis C Virus RNA, and Human Immunodeficiency Virus Types 1 and 2 RNA using the Real-Time PCR/RT-PCR method.

All components are lyophilized and ready-to use.

This product is for General Laboratory Use (GLU)

 

 

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Description

The RealLine HBV/HCV/HIV PCR assay kit is intended for differential detection of Hepatitis B Virus (HBV) DNA, Hepatitis C Virus (HCV) RNA, and Human Immunodeficiency Virus Types 1 and 2 (HIV-1 and HIV-2) RNA in blood serum (plasma) using a method based on the DNA or cDNA fragment (obtained from RNA by reverse transcription) amplification in polymerase chain reaction (PCR) with hybridization fluorescent detection in real time.

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881-R) seperately.

Sample Material

  • Blood serum (plasma)

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • HEX (535 – 554 nm) for HCV DNA fragment in RMM-1 and HIV-2 cDNA in RMM-2
  • ROX (575 – 602 nm) for HBV DNA fragment in RMM-1 and HIV-1 cDNA in RMM-2

Components

  • Ready Master Mix No. 1 (RMM-1), lyophilized – 48 test-tubes
  • Ready Master Mix No. 2 (RMM-2), lyophilized – 48 test-tubes
  • Positive Control sample (PC), lyophilized
  • Recovery Solution for Control samples (RSC)