SgrB I is a restriction enzyme purified from Streptomyces griseus with a concentration of 10 U/µl. It is an isoschizomer to Sac II. Restriction endonucleases are used in biotechnology for gene cloning, to cut DNA in smaller strands to study similarities and differences in fragment length among individuals like in RFLP (Restriction Fragment Length Polymorphism). BIORONs restriction enzymes show high purity and activity.
BamH I can be heat-inactivated after 20 min at 65°C and shows 100% activity in BIORON buffers L and K (supplied with the enzyme). Please note that activity is blocked by CpG methylation. Particular sites in lamda and phiX174 DNAs are difficult to cleave with SgrB I (as well as it’s isoschizomer Sac II).
5’…CCGC▼GG…3’
3’…GG▲CGCC…5’
