Hpa I is a restriction enzyme which is purified from an E. coli strain that carries the cloned Hpa I gene from Haemophilus parainfluenzae. It has a concentration of 10 U/µl. Restriction endonucleases are used in biotechnology for gene cloning, to cut DNA in smaller strands to study similarities and differences in fragment length among individuals like in RFLP (Restriction Fragment Length Polymorphism). BIORONs restriction enzymes show high purity and activity.
Hpa I can not be heat-inactivated and shows 100% activity in BIORON buffers A and K (supplied with the enzyme). Please note that activity is blocked by some combinations of CpG methylation.
5’…GTT▼AAC…3’
3’…CAA▲TTG…5’
