HighTaq DNA Polymerase 5 U/µl is a modified version of Taq DNA polymerase, isolated from eubacterium Thermus aquaticus strain YT-1 with a size of 94 kDa. The highly purified enzyme possesses 5′-3′ polymerisation and 5′-3′ exonuclease activity and does single 3′ dA overhangs. It is free of nonspecific endo- or exonucleases. Fragments up to 5 kb can be amplified. Special additives provide high robustness against inhibitors like polysaccarides, fats and proteins. HighTaq DNA Polymerase is suitable for common PCR applications or pathogen detection where high robustness and product yield is needed.
The polymerase is supplied with three different 10x buffers (with and without MgCl2) and additional MgCl2 for universal application on customers side.
HighTaq DNA Polymerase is also available as high concentrated variant with 75 U/µl for lyophilisation.