DFS HotTaq DNA Polymerase 5 U/µl is isolated from eubacterium Thermus aquaticus strain YT-1 with a size of 94 kDa. Blocking with antibody allows reaction setup at room temperature. The highly purified enzyme possesses 5′-3′ polymerisation and 5′-3′ exonuclease activity and does single 3′ dA overhangs. It is free of nonspecific endo- or exonucleases. Fragments up to 5 kb can be amplified. DFS HotTaq DNA Polymerase is suitable for common PCR applications or pathogen detection where high robustness and product yield is needed.
The polymerase is supplied with three different 10x buffers (with and without MgCl2) and additional MgCl2 for universal application on customers side.
