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The RealLine TBEV kit is intended for a sensitive detection of TBEV RNA in human samples or from ticks by one-step real-time RT-PCR. The results of PCR analysis are taken into account in complex diagnostics of disease.

All components are lyophilized and ready-to use.

GLU – for General Lab Use

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Description

Description

The Tick-Borne Encephalitis Virus is endemic in an area ranging from northern China and Japan, through far-eastern Russia to Europe. TBEV is transmitted by the bite of infected ticks (Ixodes scapularis, Ixodes ricinus and Ixodes persulcatus), or (rarely) through the non-pasteurized milk of infected cows, sheeps and goats. TBE is a viral infectious disease involving the central nervous system (CNS). The disease is incurable once manifested, so there is no specific drug therapy for TBE.

Sample Material

  • Blood serum, plasma
  • Cerebrospinal fluid
  • Tick suspension sample

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for TBEV RNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881-R) seperately.

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON GmbH)

CFX96, iQ5 iCycler (Bio-Rad, USA)

DT96, DTLite (DNA-Technology, Russia)

Our kits are compatibel to other Real-Time cyclers. Please read the IFU properly and ask us for recommendations of device settings!

Additional information

Additional information

RealLine TBEV FSME

Fla, Str

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