RealLine Chlamydia trachomatis / Neisseria gonorrhoeae

This product is available on request. Please contact us.

The RealLine Chlamydia trachomatis / Neisseria gonorrhoeae kits are designed for a differential and sensitive detection of  the plasmid and genomic DNA of Chlamydia trachomatis and Neisseria gonorrhoeae DNA in human samples by Real-Time PCR. The results of PCR analysis are taken into account in complex diagnostics of disease.

All components are lyophilized and ready-to use.

GLU – for General Lab Use

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Description

Description

Untreated infections by Chlamydia trachomatis can cause serious reproductive and other health problems with both short-term and long-term consequences. In both sexes Chlamydia infection can cause proctitis, trachoma and infertility as well as prostatis, epidiymitis in men and cervictis, PID, ectopic pregnancy and acute/chronic pelvic pain in women. Neisseria gonorrhoeae are gram-negative motile bacteria and the causative agent of gonorrhea, one of the most common sexually transferred disease. In women gonorrhea can lead to severe symptoms such as infertility and abortion. In men gonorrhoeae can lead to painful urethritis. Detection Channels

    • FAM (495 – 520 nm) for Internal Control (IC)
    • HEX (535 – 554 nm) for Chlamydia trachomatis DNA
    • ROX (575 – 602 nm) for Neisseria gonorrhoeae DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881-R) seperately.

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON GmbH)

CFX96, iQ5 iCycler (Bio-Rad, USA)

DT96, DTLite (DNA-Technology, Russia)

Our kits are compatible with other Real-Time cyclers. Please read the IFU properly and ask us for recommendations of device settings!

Additional information

Additional information

RealLine STI Multiplex

Str

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