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DFS HotTaq DNA Polymerase

DFS HotTaq DNA Polymerase is the optimized mixture of DFS-Taq DNA Polymerase and Anti-Taq monoclonal antibodies. Antibodies block polymerase activity during set-up of the PCR reactions at ambient temperature. The inhibition of DFS HotTaq DNA polymerase is completely reversed when the temperature is above 70 °C.
The DFS HotTaq Polymerase catalyzes the polymerization of nucleotides into duplex DNA in 5’ – 3’ direction in the presence of magnesium ions and possesses a 5’ – 3’ exonuclease activity.
The DNA-free Taq Polymerase is highly purified and is free of nonspecific endo- or exonucleases.

Product Cat.No. Packsize Quantity  
DFS HotTaq DNA Polymerase , 5 U / µl
[PDS]
101004 500 U Add to cart
DFS HotTaq DNA Polymerase , 5 U / µl
101024 2500 U Add to cart

Features

  • Free from bacterial DNA
  • Universal buffer system for different applications
  • High sensitivity
  • Efficient amplification of single copy genes
  • High product yield
  • Available as high concentrated customized product with reduced glycerin concentration, suitable for lyophilization

Applications

  • Amplification of DNA fragments up to 5 kb
  • Amplification of single-copy genes
  • Labelling of PCR products with modified nucleotides (biotin-dUTP, fluorescein-dUTP)
  • Cycle sequencing
  • Generation of PCR products for TA-cloning
  • Detection of bacterial pathogens