Reverse Transcription

Reverase^TM is a modified enzyme purified from E.coli strain harbouring a plasmid that directs the synthesis of Moloney Murine Leukemia virus (M-MuLV) reverse transcriptase. Reverase is an RNA or DNA directed DNA polymerase.
The enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single stranded DNA as a template. This enzyme had been genetically altered to remove associated RNase H activity. Removal of the RNase H activity resulted in an increase of full-length cDNA products.

Features

• No RNase H activity
• Resulted cDNA can be amplified by PCR with Taq Polymerase

Applications

• First strand synthesis of cDNA from RNA molecules
• Detection of viruses in combination with PCR amplification of cDNA