WGA

Single Cell WGA Kit

BIORON´s Single Cell WGA Kit, developed and produced at BIORON GmbH, is an improved version of the DOP (degenerated oligo PCR) with optimized primer sequences and BIORON’s unique SD Polymerase. This enzyme combines the strand displacement capability with heat stability up to 92 °C. Due to these features it is possible to use this enzyme for both amplification methods without additional pipetting or hands on time. The Kit contains everything that is necessary for amplification of human genomic DNA from single cells or low copy templates.

The amplification is based on a multiple displacement reaction in combination with specially designed degenerated primers optimized for human genomic DNA. The SD Polymerase is able replace former strands during the synthesis of the new strand. In combination with the temperature stability up to 92 °C the two common reaction steps within a whole genome amplification assay does not need additional hands‑on time. This minimizes the risk of contamination and handling errors.

Each Kit contains a 2.5x MM (Master Mix without enzyme); 10 U/μl SD Polymerase; Lysis Buffer and PCR grade water. The Lysis is designed to give best results when carried out in minimal volume. The 2.5x Mastermix can be added directly after the Lysis together with the SD-Polymerase and amplification occurs in the same tube.

Product Cat.No. Packsize Quantity  
Single Cell WGA

[IFU]
118710 10 rcs Add to cart
Single Cell WGA

118750 1 x 50 rcs Add to cart

see also: SD Polymerase

Features

  • Amplification finished in less than 2 hours
  • Different genomic amplification coverage than other enzymes
  • Long fragments up to 3 Kb
  • One Enzyme for the whole genome amplification

Applications

  • Single cell DNA analysis
  • Next Generation Sequencing (NGS)
  • PID
  • SNP Genotyping
  • Allel Dropout
  • qPCR- and PCR-based mutation detection
  • STR and RFLP analysis
  • Array technologies (comparative genomic hybridization)

WGA Kit 2020.jpg