Special Application

SNPase Hotstart

SNPase HotStart DNA Polymerase is the HotStart version of Klen SNPase. It is designed for cycle sequencing with dideoxynucleotides in automated fluorescent DNA sequencing as well as for manual DNA sequencing with 35S label. This enzyme has only 5 ‘- 3’ polymerase activity and is also recommended for SNP genotyping by allele-specific PCR (AS-PCR), allele-specific primer extension (AS-PEX) and minisequencing procedures.
The relative mutation rate during polymerization is 10-15 fold lower for Klen SNPase compared with Taq DNA polymerase

Product Cat.No. Packsize Quantity  
SNPase Hotstart, 20 U / µl
[PDS]
108210 500 U Add to cart
SNPase Hotstart, 20 U / µl
108250 2500 U Add to cart

Features

  • High fidelity of dNTPs and ddNTPs incorporation
  • High specificity leads to low level of background PCR

Applications

  • Cycle sequencing with dideoxynucleotides in automated fluorescent DNA sequencing or manual DNA sequencing
  • SNP genotyping with AS-PCR, AS-PEX and minisequencing procedures