Special Application

SD Polymerase

SD Polymerase is a novel artificial thermostable polymerase providing a strong strand displacement activity. Unlike natural enzymes with strong strand displacement activity as Phi29 or Bst Polymerase, which are active below 68 °C only, SD Polymerase is stable up to 93 °C. This allows running isothermal amplification like LAMP with initial heating-up for increased specificity.
SD Polymerase has 5’ – 3’ polymerase and 5’ – 3’ strand displacement activities. It does not have any exonuclease activity. The enzyme does 3’-A-overhangs. SD Polymerase is also available as a HotStart version.

Product Cat.No. Packsize Quantity  
SD Polymerase, 10 U / µl
[PDS]
108702 200 U Add to cart
SD Polymerase, 10 U / µl
108710 1000 U Add to cart
SD Polymerase, 50 U / µl
[PDS]
108800 1000 U Add to cart
SD Polymerase, 50 U / µl
108850 5000 U Add to cart
SD Polymerase Hotstart, 10 U / µl
[PDS]
108902 200 U Add to cart
SD Polymerase Hotstart, 10 U / µl
108910 1000 U Add to cart

SD Polymerase is used in our WGA-Kit for whole genome amplifcation:  One Step WGA Kit

Features

  • Available in high and low concentrations and as a HotStart version
  • Higher yields, speed and efficiency compared to Taq Polymerase
  • Suitable for amplification of short (100 bp) and long (20 – 30 kb) DNA fragments
  • Suitable for simple (e.g. plasmids) and complex (e.g. genomic DNA) templates

Applications

  • Suitable for different PCR methods, also long-range PCR
  • PCDR (Polymerase Chain Displacement Reaction)
  • Strand displacement like LAMP (Loop-mediated isothermal amplification)

Publications

2014 GenomeWeb

2014 Ignatov et al.

2017 Shagin et al.

2017 Blagodatskikh et al.

2017 Smith

2017 Shchit et al.

2018 Alyethodi et al.

2018 Lou et al.

2018 Wang et al. (Manuscript)