Special Application

Klen SNPase

Klen SNPase DNA Polymerase is an optimized version of thermostable DNA polymerase designed for cycle sequencing with dideoxynucleotides in automated fluorescent DNA sequencing as well as for manual DNA sequencing with 35S label. This enzyme has only 5 ‘- 3’ polymerase activity and is also recommended for SNP genotyping by allele-specific PCR (AS-PCR), allele-specific primer extension (AS-PEX) and minisequencing procedures.
The relative mutation rate during polymerization is 10-15 fold lower for Klen SNPase compared with Taq DNA polymerase.
For HotStart applications please use SNPase HotStart (Ref. No. 1082010, 108250).

Product Cat.No. Packsize Quantity  
Klen SNPase, 25 U / µl
[PDS]
108205 500 U Add to cart
Klen SNPase, 25 U / µl
108225 2500 U Add to cart

Applications

  • Automated fluorescent DNA sequencing and manual DNA sequencing
  • Highly suited to SNP genotyping by AS-PCR, AS-PEX and minisequencing

Features

  • High specificity and very low background in AS-PEX, AS-PCR and minisequencing
  • High–fidelity incorporation of deoxy- and dideoxynucleotides