Special Application

DF-Pfu DNA Polymerase

DF-Pfu DNA Polymerase, isolated from the hyperthermophilic archae bacteria Pyrococcus furiosus is a thermostable Polymerase of approximately 92 kDa. The enzyme replicates DNA at 75 °C, catalyzing the polymerization of nucleotides into duplex DNA in the 5’ → 3’ direction in the presence of magnesium (prefers MgSO4).
Because of proofreading activity DF-Pfu DNA Polymerase-generated PCR fragments will exhibit the lowest error rate of any thermostable DNA polymerase, a 12-fold increase in fidelity of DNA synthesis compared with Taq DNA Polymerase (error rate 0.2 × 10-5).

Product Cat.No. Packsize Quantity  
DF-Pfu DNA Polymerase, 5 U / µl
[PDS]
108105 500 U Add to cart
DF-Pfu DNA Polymerase, 5 U / µl
108125 2500 U Add to cart

Features

  • 3’ to 5’ exonuclease proofreading activity
  • Generates blunt-ended DNA-fragments
  • Free of bacterial DNA

Applications

  • PCR and primer extension reactions which require high-fidelity synthesis
  • Blunt-end ligation
  • Precise amplification of bacterial sequences