Hotstart

AptaHotTaq Polymerase

AptaHotTaq is an optimized mixture of thermostable Taq DNA polymerase and DNA-aptamers. The enzyme activity is blocked by aptamers at room temperature. The aptamers dissociate reversible from the polymerase, when temperature is above 50 °C.
AptaHotTaq possesses a 5’ – 3’ polymerase activity and a 5’ – endonuclease activity. It is suitable for qPCR applications, because it minimizes formation of primerdimers and reduces unspecific oligonukleotide priming. Therefore amplification of the DNA-target is increased.
AptaHotTaq can be used in usual PCR protocols. So a separate incubation step is not necessary, but it can be helpful to dissolve the secondary structure of the template DNA so amplification is not hindered.

Product Cat.No. Packsize Quantity  
AptaHotTaq DNA Polymerase, 30 U / µl
[PDS]
119630 2000 U Add to cart

Features

  • Highly active HotStart Taq Polymerase
  • DNA-aptamer blocked
  • Activation of enzyme at temperature above 50 °C

Application

  • Real-Time PCR
  • High throughput applications
  • For diagnostic use
  • AptaHotTaq 30 U/µl can be used for lyophilization