endecnplse

Sfr274 I

Catalog #Pack sizePrice, EuroSHOP
2450202000 u24,00Add to cart
24510010000 u96,00Add to cart

Opens internal link in current windowDownload: Datasheet Sfr274 I (Xho I)


C↓TCGAG

GAGCT­↑C

Purified from Streptomyces fradiae 274

Isoschizomer Xho I

Concentration:
50000 units/ml

Activity assay:
Phage λ DNA/PvuI digest, SE buffer B, 37oC.

Recommended buffer:
B

Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 5 units of the enzyme at 37oC.

Heat inactivation (20 min, 65oC):
yes

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