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Bme18 I

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Opens internal link in current windowDownload: Datasheet Bme18 I (Ava II)


G↓GWCC

CCWG­↑G

Purified from Bacillus megaterium 18

Isoschizomer Ava II

Concentration:
10000 units/ml.

Activity assay:
Phage λ DNA, SE buffer O, 37oC.

Recommended buffer:
O

Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 20 units of enzyme at 37oC.

Heat inactivation (20 min, 65oC):
Yes

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