Afe I
| Catalog # | Pack size | Price, Euro | SHOP |
| 207002 | 200 u | 104,00 | Add to cart |
| 207010 | 1000 u | 416,00 | Add to cart |
Download: Datasheet Afe I (Eco47 III)
AGC↓GCT
TCG↑CGA
Purified from Alcaligenes faecalis T2774
Isoschizomer Eco47 III
Concentration:
10-50000 units/ml
Activity assay:
Phage λ DNA/BamHI digest, SE buffer Y, 37oC.
Recommended buffer:
Y
Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 80% of the DNA fragments may be ligated and recut.
Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 10 units of enzyme at 37oC.
Heat inactivation (20 min, 65oC):
Yes
