SnaB I
| Catalog # | Pack size | Price, Euro | SHOP |
| 250136S | 350 units | 20,00 | Add to cart |
| 250136L | 1.750 units | 98,00 | Add to cart |
Cutting sequence: TAC↓GTA
Isoschizomere: N/A
Source: Sphaerotilus natans
Buffer supplied: 10x SnaB I.
Substrate for unit definition: λ DNA (EcoRI digest).
Reaction conditions: 10 mM Bis Tris Propane-HCI (pH 7.0 at 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37°C.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20°C.
Absence of contaminants: Ten units of SnaB I do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA/EcoR I digest at 37°C. After ten-fold overdigestion with SnaBI, more than 98% of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: 80°C for 20 minutes.
Star activity: Star activity is observed at a greater than 160-fold overdigestion with SnaBI.
Reaction Buffer Table
