Sau3A I
| Catalog # | Pack size | Price, Euro | SHOP |
| 250147S | 500 units | 20,00 | Add to cart |
| 250147L | 2.500 units | 98,00 | Add to cart |
Cutting sequence:
Sau3A I (isoschizomer) ↓GATC
Source: Streptomyces species
Buffer supplied: 10x M
Substrate for unit definition: λ DNA.
Reaction conditions: 50 mM NaCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 10 mM MgCl2, 1mM dithiothreitol, 100 μg/ml BSA. Incubate at 37°C.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20oC.
Absence of contaminants: 50 units of Sau3A I do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA at 37oC. After 50-fold overdigestion with Sau3A I, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: 65oC for 20 minutes.
Reaction Buffer Table
