Nru I
| Catalog # | Pack size | Price, Euro | SHOP |
| 250125S | 700 units | 20,00 | Add to cart |
| 250125L | 3.500 units | 98,00 | Add to cart |
Cutting sequence: TCG↓CGA
Isoschizomere: N/A
Source: Nocardia rubra.
Buffer supplied: 10x Nru I.
Substrate for unit definition: λ DNA.
Reaction conditions: 100 mM KCI, 50 mM Tris-HCl (pH 8.0 at 25°C), 10 mM MgCl2, 100 μg/ml BSA. Incubate at 37°C.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20°C.
Absence of contaminants: Fifty units of NruI do no produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA at 37°C. After ten-fold overdigestion with NruI, less than 20% of the DNA fragments can be ligated.
Heat inactivation: 65°C for 20 minutes.
Star activity: Large excess of the enzyme results in the appearance of star activity.
Reaction Buffer Table
