EcoR I
| Catalog # | Pack size | Price, Euro | SHOP |
| 250114S | 5.000units | 12,00 | Add to cart |
| 250114L | 25.000 units | 43,50 | Add to cart |
Cutting sequence: G↓AATTC
Isoschizomere: N/A
Source: E. coli RY 13.
Buffer supplied: 10x EcoR I.
Substrate for unit definition: λ DNA.
Reaction conditions: 50 mM NaCl, 100 mM Tris-HCl (pH 7.9 at 25°C), 5 mM MgCl2, 0.025% Triton X-100, 100 μg/ml BSA. Incubate at 37°C.
Storage buffer: 300 mM NaCl, 5 mM KPO4 (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 0.15% Triton X-100, 200 μg/ml BSA and 50% glycerol. Store at -20°C.
Absence of contaminants: Ten units of EcoRI do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA at 37°C. After 50-fold overdigestion with EcoRI, greater than 98% of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: 65°C for 20 minutes.
Star activity: Conditions of low ionic strength, high enzyme concentration, glycerol concentration>5%, or pH>8.0 may result in star activity.
Reaction Buffer Table
