BstE II
| Catalog # | Pack size | Price, Euro | SHOP |
| 250144S | 1.750 units | 20,00 | Add to cart |
| 250144L | 8.750 units | 98,00 | Add to cart |
Cutting sequence: G↓GTNACC
Source: Bacillus stearothermophilus.
Buffer supplied: 10x BstE II
Substrate for unit definition: λ DNA
Reaction conditions: 100 mM KCl, 10 mM Tris-HCl (pH 7.4 @ 25°C), 5 mM MgCl2, 1 mM dithiothreitol, 0.1% Triton X-100, 100 μg/ml BSA. Incubate at 60oC under parafin oil.
Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20oC.
Absence of contaminants: 150 units of BstEII do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA at 60oC. After 100-fold overdigestion with BstEII, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: No.
Note: BstE II exhibits 10-15% activity at 37°C.
Reaction Buffer Table
