BssA I (BsrF I, Cfr10 I)
| Catalog # | Pack size | Price, Euro | SHOP |
| 250107S | 650 units | 20,00 | Add to cart |
| 250107L | 3250 units | 98,00 | Add to cart |
Cutting sequence: (A/G)↓CCGG(T/C)
Isoschizomere: Cfr10 I, BsrF I
Source: Bacillus species.
Buffer supplied: 10x BssA I.
Substrate for unit definition: λ DNA.
Reaction conditions: 100 mM KCl, 20 mM Tris-HCl (pH 8.5 at 25°C), 3 mM MgCl2, 0.04% Triton X-100, 100 μg/ml BSA. Incubate at 65°C under parafin oil.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20°C.
Absence of contaminants: Fifty units of BssAI do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA at 65°C. After 30-fold overdigestion with BssAI, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Low salt concentration or large excess of enzyme results in the appearance of star activity.
Heat inactivation: No.
Reaction Buffer Table
