T4 RNA Ligase
| Catalog # | Pack size | Price, Euro | SHOP |
| 404010 | 1000 u | 58,60 | Add to cart |
| 404050 | 5000 u | 263,70 | Add to cart |
Datasheet: T4 RNA Ligase
Description::
T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA . Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates. The enzyme has a molecular weight of 43.5kDa.
Source:
Purified from E. coli strain containing the plasmid with T4 ligase gene.
Applications:
- Labeling of 3´ -termini of RNA with 5´ -[32P] pCp
- Inter- and intramolecular joining of RNA and DNA molecules
- Synthesis of single-stranded oligodeoxyribonucleotides
- Incorporation of unnatural amino acids into proteins
Reaction Conditions:
1X T4 RNA Ligase Buffer: 50 mM Tris-HCl (pH 7.8), 10 mM MgCl2, 10 mM DTT and 1 mM ATP. Incubate at 37°C.
Unit Definition:
One unit is defined as the amount of enzyme required to convert 1 pmol of AMP to an acid-insoluble form in 10 minutes at 25°C.
Concentration:
10-100,000 units/ml.
Heat Inactivation:
65°C for 15 minutes or boiling for 2 minutes.
