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X-gal (MBG)

Catalog #Pack sizePrice, EuroSHOP
4065011 g60,00Add to cart
4065055 g260,00Add to cart
40652510 g480,00Add to cart

 

Datasheet: X-gal

Description:

X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) is a noninducing chromogenic substrate for beta-galactosidase, which hydrolyzes X-Gal forming an intense blue precipitate. X-GaI is most frequently used in conjunction with IPTG in blue/white colony screening to detect recombinants (white) from non-recombinants (blue). It is also utilized for selection of beta-galactosidase reporter gene activity in transfection of eucariotic cells and for detection of beta-galactosidase in immunology and histochemistry applications.

Quality Control:

Purity of >98% by HPLC. Tested in the blue/white colony screening experiment.

Storage:
Store at -20°C in the dark.

Shipment:
room-temperature

Formula: C14H15BrClNO6

Molecular Weight:
408.6         

Melting Point:
223°C  

Reference:
1. Sambrook, J., Russell, D.W., Molecular Cloning: A Laboratory Manual, the Third edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1.124-1.125, A1.27, 2001.

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