• Home
• Contact
• Product finder
• Advertising Material
• Distribution
All Products

BIORON   » All Products   » Diagnostic Kits   » R&D-Line freeze dryed form    » Viral RNA detection by RT-PCR Assay

• PCR
• Devices
• Diagnostic Kits
  • CE-line
  • R&D-Line freeze dryed form
  • S-Taq Polymerase
  • DNA Standards
• DNA/DNA Markers
• DNA-RNA Purification
• Protein Purification
• Dialysis Membranes
• Other Enzymes
• Mol-Bio Chemicals
• Restriction Endonucleases
• Plasticware

Download
Catalog 2007

Introduction:
GenePak RNA PCR test kits are designed for the non-quantitative detection of viral RNA in biosamples (plasma, serum, whole blood, swabs, epithelium cells etc.). The time for the analysis is close to 5 hours. The kit is designed for 100 reactions including 10 positive and 10 negative (80 tests for DNA and reverase transcription and 10 + 10 tubes for positive and negative control) control reactions.

Basic principles of detection
The kit is based on 2 basic techniques – Reverse Transcription and PCR. The kits contain tubes with lyophilized MasterMixes Ready-To-Use for Reverse- Transcription –PCR starting from the purified RNA.
The kits contain random primers for Reverse Transcription initiation, M-MuLV Reverse Transcriptase, Placental Ribonuclease Inhibitor, Taq DNA polymerase and specific primers for PCR. The cloned fragments of viral cDNA with the conservative sequences are used as positive controls.
PCR fragments detection should be done by UV-light after agarose gel electrophoresis with the EtdBr. The fragment of DNA with the specific size indicates the presence of the corresponding RNA template in the biosample.

download Datasheet/manual for: DRY-Mix Gene Pack for infection diseases (RNA)

The RNA test system contains:
- 80 PCR test tubes (colorless)
- 80 rt-mastermix test tubes for reverase transcription (colorless)
- 10 PCR positive controls (red stained)
- 10 PCR negative controls (blue stained)
- in total: 180 tubes with lyophilized reagents (1 tube – 1 reaction)
- RT stop solution (one Tube, 1ml)
- RT diluent (one Tube, 1 ml)
- PCR diluent (one Tube, 1ml)

Please note: The kits are not CE-marked

Copyright © BIORON GmbH