endecnplse

Sfi I

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244005500 u20,00Add to cart
2440252500 u80,00Add to cart

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GGCCNNNN↓NGGCC

CCGGN­↑NNNNCCGG

Purified from Streptomyces fimbriatus

Concentration:
2000-15000 units/ml

Activity assay:
Adenovirus DNA, SE buffer G, 50oC

Recommended buffer:
G

Ligation/recutting assay:
After 3-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 2 units of the enzyme at 50oC.

Heat inactivation (20 min, 65oC):
No

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