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Site-specific Nickase NBst9

Catalog #Pack sizePrice, EuroSHOP
257001100 u30,00Add to cart
257005500 u120,00Add to cart

Download: Datasheet Nickase NBst9


GAGTCNNNN↓NN

CTCAGNNNNNNN

Purified from Bacillus stearothermophilus

Concentration:
2-10000 units/ml

Activity assay:
Phage T7 DNA, SE buffer N.BstT9, 55oC

Recommended buffer:
N.BstT9

Ligation/recutting assay:
After 5-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Heat inactivation: 20 min, 65oC - No;  20 min, 80oC - Yes

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