endecnplse

Bse118 I

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271002200 u40,00Add to cart
2710101000 u160,00Add to cart

Opens internal link in current windowDownload: Datasheet Bse118 I (Cfr10 I)


R↓CCGGY

YGGCC­↑R   

Purified from Bacillus stearothermophilus 118

Isoschizomer Cfr10 I

Concentration:
2000 units/ml

Activity assay
Phage λ DNA, SE buffer O, 65oC.

Recommended buffer:
O

Ligation/recutting assay:      
After 2-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and  recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 2 units of enzyme at 65oC.

Heat inactivation (20 min, 65oC) :
No

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