endecnplse

AsuNH I

Catalog #Pack sizePrice, EuroSHOP
211005500 u48,00Add to cart
2110252500 u192,00Add to cart

Opens internal link in current windowDownload: Datasheet AsuNH I (Nhe I)


G↓CTAGC
 

CGATC­↑G

Purified from Actinobacillus suis NH

Isoschizomer Nhe I

Concentration:
5-20000 units/ml

Activity assay:
Phage λ DNA/Hind III digest, SE buffer B, 37oC.

Recommended buffer:
B

Ligation/recutting assay:
After 5-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:   
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 10 units of the enzyme at 37oC.

Heat inactivation (20 min, 65oC):
Yes

to topprint